|
| Status |
Public on Oct 06, 2006 |
| Title |
CD11b_Tumor bearing at time 0_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
CD11b+ sorted cells from mouse spleen
|
| Organism |
Mus musculus |
| Characteristics |
BALB/c
Gender: female
Age: 8-12 weeks
Tissue: spleen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by TRIzol (Invitrogen) from CD11b+ sorted cells according to the manufacturer?s instructions with little modifications. The quality and quantity of RNA sample were determined by Agilent RNA 6000 Nano Chip (Agilent Technologies). cDNA from purified total RNA was produced by M-MLV reverse transcriptase (Invitrogen) following manufacturer protocol. All the pre-hybridization quality controls were performed with the Agilent 2100 bioanalyzer (Agilent Technologies).
|
| Label |
Biotin
|
| Label protocol |
5 μg of total RNA were used to generate double-stranded cDNA (Invitrogen). Synthesis of Biotin-labeled cRNA was performed using the BioArrayTM HighYieldTM RNA Transcript Labeling Kit (ENZO Biochem).
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures (see GeneChip? Expression Analysis Technical Manual)
|
| Scan protocol |
Standard Affymetrix procedures (see GeneChip? Expression Analysis Technical Manual)
|
| Description |
Global transcriptional analysis to explore the gene expression pattern of CD11b purified from the mouse spleen (tumor free and tumor bearing mice)
|
| Data processing |
Affymetrix MAS5.0 procedure
|
| |
|
| Submission date |
Aug 04, 2006 |
| Last update date |
Oct 06, 2006 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
[email protected]
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5455 |
Gene expression profiling of CD11b purified from mouse spleen (tumor-free and tumor-bearing mice) |
|
