|
| Status |
Public on Apr 29, 2014 |
| Title |
S2 GFP dsRNA 3 |
| Sample type |
RNA |
| |
|
| Source name |
S2 cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: S2 cells
treated with: GFP dsRNA
|
| Treatment protocol |
1.2 million exponentially growing S2 cells were treated with 20µg dsRNA in 1 ml of serum free media on 6-well plates. Serum containing media was added 1h later for a final FBS concentration of 10%. Cells were cultured for 72h before sample collection.
|
| Growth protocol |
Drosophila S2 cells were cultured in Drosophila SFM media (Gibco) supplemented with 10% FBS, L-glutamine and antibiotics
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA, Qiagen RNeasy Plus Mini Kit according to manufcturers protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 4µg total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual P/N 702232 Rev.2)
|
| |
|
| Hybridization protocol |
10 µg of fragmented cRNA were hybridized on GeneChip Drosophila Genome 2.0 arrays according to Affymetrix standars protocols (Expression Analysis Technical Manual P/N 702232 Rev.2). Affymetrix Fluidics station 450 was used for washing and staining.
|
| Scan protocol |
GeneChips were scanned with Affymetrix 3000 scanner
|
| Description |
GFP_3
|
| Data processing |
GeneSpring GX 12.1 GC-RMA
|
| |
|
| Submission date |
Nov 13, 2013 |
| Last update date |
Apr 29, 2014 |
| Contact name |
Emilia Kuuluvainen |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Helsinki
|
| Department |
Institute of Biotechnology
|
| Street address |
Viikinkaari 9
|
| City |
Helsinki |
| ZIP/Postal code |
00790 |
| Country |
Finland |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE52343 |
Cdk8, Cyclin C, Med12 or Med13 depletion effect on gene expression in Drosophila S2 cells |
|
