|
| Status |
Public on Jan 01, 2015 |
| Title |
n5 dnNrf2 (wt) |
| Sample type |
RNA |
| |
|
| Source name |
wt 3
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wt
age: P2.5
tissue: skin
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Mouse skin was collected at newborn mice at P2.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol-based total RNA extraction
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were labeled with Cy3 according to Agilent's labeling protocol
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed at 55°C for 20 hours in Agilent's microarray hybridization chambers, followed by wash procedures according to the manufacturer’s recommended protocols
|
| Scan protocol |
Microarray was scanned in an Agilent scanner at 5μm resolution, and the array data was extracted with Agilent feature extraction software using the miRNA_105_Jan09 protocol
|
| Description |
n5 dnNrf2 (wt)
|
| Data processing |
Agilent Feature Extraction Software (version 10.7.3.1) was used.
|
| |
|
| Submission date |
Nov 18, 2013 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Svitlana Kurinna |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Manchester
|
| Street address |
Dover Street
|
| City |
Manchester |
| ZIP/Postal code |
M13 9PT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL15547 |
| Series (1) |
| GSE52454 |
Functional analysis of the cytoprotective transcription factor Nrf2 in keratinocytes: Identification of Nrf2-regulated miRNAs |
|
