|
| Status |
Public on Feb 05, 2014 |
| Title |
WT_anti_Fkh_rep_1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Fkh ChIP DNA from WT cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: ura3::BrdU‐Inc (URA3) bar1Δ::hisG
strain: CVy43
chip antibody: anti-Fkh polyclonal
|
| Growth protocol |
cells were grown in YEPD
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted as described in as described in Viggiani CJ, Aparicio JG, Aparicio OM (2009) ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays. Methods Mol Biol 521: 255-278.
|
| Label |
Cy5
|
| Label protocol |
Nimblegen standard protocol
|
| |
|
| Channel 2 |
| Source name |
Total DNA from WT cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: ura3::BrdU‐Inc (URA3) bar1Δ::hisG
strain: CVy43
chip antibody: anti-Fkh polyclonal
|
| Growth protocol |
cells were grown in YEPD
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted as described in as described in Viggiani CJ, Aparicio JG, Aparicio OM (2009) ChIP-chip to analyze the binding of replication proteins to chromatin using oligonucleotide DNA microarrays. Methods Mol Biol 521: 255-278.
|
| Label |
Cy3
|
| Label protocol |
Nimblegen standard protocol
|
| |
|
| |
| Hybridization protocol |
Nimblegen standard protocol
|
| Scan protocol |
Genepix Personal 4100A Scanner (Axon Instruments).
|
| Description |
α-Fkh ChIP-chip of WT
|
| Data processing |
We used RINGO package (http://www.biomedcentral.com/1471-2105/8/221) in BIOCONDUCTOR suite to perform normalization.
|
| |
|
| Submission date |
Nov 18, 2013 |
| Last update date |
Feb 05, 2014 |
| Contact name |
Andrew Zachary Ostrow |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Southern California
|
| Department |
Biological Sciences
|
| Lab |
Oscar Aparicio
|
| Street address |
1050 Childs Way
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platform ID |
GPL14887 |
| Series (1) |
| GSE52467 |
Fkh1 and Fkh2 Bind Multiple Chromosomal Elements in the S. cerevisiae Genome with Distinct Specificities and Cell Cycle Dynamics |
|
