|
| Status |
Public on Nov 21, 2013 |
| Title |
293 transfected with Aire replicate #2 |
| Sample type |
RNA |
| |
|
| Source name |
293 transfected with Aire
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK 293
cell type: human embryonic kidney
|
| Treatment protocol |
HEK 293 cells were transfected using Mirus 293 transfection reagent following manufacturers protocol.
|
| Growth protocol |
Transfected HEK 293 cells were grown for 72hrs in complete DMEM prior to RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Qiagen RNAeasy mini kit according to manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
NuGEN Ovation Pico WTA V2 and NuGEN Encore Biotin Module
|
| |
|
| Hybridization protocol |
See manufacturer protocol
|
| Scan protocol |
GCS3000
|
| Description |
293 cells transfected with Aire only biologic replicate #2
|
| Data processing |
AGCC 3.2.2 Microarrays were normalized for array-specific effects using Affymetrix's Robust Multi-Array (RMA) normalization
|
| |
|
| Submission date |
Nov 20, 2013 |
| Last update date |
Nov 21, 2013 |
| Contact name |
Mark Stuart Anderson |
| E-mail(s) |
[email protected]
|
| Organization name |
UCSF
|
| Department |
Diabetes Center
|
| Lab |
Anderson Lab
|
| Street address |
513 Parnassus Avenue, HSW Room 1102, Box 0540
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE52546 |
Gene expression of HEK 293 cells transfected with Vector (PCMV), Aire, or MBD-VP16 |
| GSE52548 |
Aire co-opts the repressive ATF7ip/MBD1 protein complex for the induction of immune tolerance |
|
