|
| Status |
Public on Dec 01, 2014 |
| Title |
HCC of STAM mice 2-2 |
| Sample type |
RNA |
| |
|
| Source name |
HCC of STAM mice
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL6
strain: STAM (NASH-HCC model)
tissue: Hepatocellular carcinoma (HCC)
|
| Treatment protocol |
STAM mice develop HCC at 18 weeks of age.
|
| Growth protocol |
STAM mice, a NASH-HCC model, were injected with STZ and fed with high-fat diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using miRNesy mini kit (Qiagen).
|
| Label |
Hy5
|
| Label protocol |
Extracted total RNA was labeled with Hy5 using the miRCURY LNA Array miR labeling kit (Exiqon, Vedbaek, Denmark)
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
HCC2-2
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals. Measurements of both duplicate spots with the signal intensities greater than 2 standard deviations (SD) of the background signal intensity were considered to be valid. A relative expression level of a given miRNA was calculated by comparing the signal intensities of the averaged valid spots with their mean value throughout the microarray experiments.
|
| |
|
| Submission date |
Nov 28, 2013 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16362 |
| Series (2) |
| GSE52821 |
miRNA expression profiles in the liver of a NASH-HCC model mice |
| GSE52822 |
Aberrant expression of miRNAs in the liver carcinogenesis from NASH |
|
