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Sample GSM129270 Query DataSets for GSM129270
Status Public on Jan 18, 2008
Title NICD Injected 2
Sample type RNA
 
Source name Animal pole explant
Organism Xenopus laevis
Characteristics Wild type, NICD RNA Injected Stage 20 explant
Extracted molecule total RNA
Extraction protocol Animal pole tissue explants were isolated embryonic stage 8 and cultured until sibling embryos reached stage 20.
Label biotin
Label protocol 1 µg of total RNA was processed using MessageAmp™ aRNA Amplification Kit (AMBION) to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description St 20, NICD injected animal pole tissue explant
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Aug 18, 2006
Last update date Aug 09, 2007
Contact name Curtis R Altmann
E-mail(s) [email protected]
Phone 8506442925
Organization name Goodwin Procter LLP
Street address 599 Lexington Avenue
City New York
State/province NY
ZIP/Postal code 10022
Country USA
 
Platform ID GPL1318
Series (1)
GSE5564 Notch Signaling in Glial Development

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 145.2 P 0.010317
AFFX-BioB-M_at 218.6 P 0.000195
AFFX-BioB-3_at 159.8 P 0.001248
AFFX-BioC-5_at 408.3 P 0.000147
AFFX-BioC-3_at 246.4 P 0.000147
AFFX-BioDn-5_at 263.1 P 0.00007
AFFX-BioDn-3_at 1762.6 P 0.00011
AFFX-CreX-5_at 4496.7 P 0.000044
AFFX-CreX-3_at 9438.1 P 0.000044
AFFX-DapX-5_at 23.2 A 0.287743
AFFX-DapX-M_at 26.2 A 0.41138
AFFX-DapX-3_at 6.3 A 0.932322
AFFX-LysX-5_at 20.1 A 0.574038
AFFX-LysX-M_at 7.3 A 0.631562
AFFX-LysX-3_at 16.6 A 0.51489
AFFX-PheX-5_at 3.1 A 0.843268
AFFX-PheX-M_at 3.9 A 0.860518
AFFX-PheX-3_at 30.4 A 0.51489
AFFX-ThrX-5_at 12 A 0.617401
AFFX-ThrX-M_at 3.6 A 0.897835

Total number of rows: 15611

Table truncated, full table size 504 Kbytes.




Supplementary file Size Download File type/resource
GSM129270.EXP.gz 481 b (ftp)(http) EXP

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