|
| Status |
Public on Mar 31, 2009 |
| Title |
E_C_Gad_sample3 |
| Sample type |
RNA |
| |
|
| Source name |
embryo culture 5-7 days, 2.1 kb Gad1(s)-RFP
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Strain: 2.1 kb Gad1(s)-RFP
Age: Adults for collection were 2 weeks old
Tissue: Embryo culture
|
| Treatment protocol |
Embryo collection and culture were performed as previously described (Salvaterra, P.M., Hayashi, I., Perez-Magallanes, M., & Ikeda, K. (2006) Primary culture of Drosophila embryo cells. in: Cell and Tissue Culture: Assorted Techniques, Elsevier Science USA, pp. 151-155). Cultures were initiated by dissociating early gastrula stage embryos prior to the differentiation of any neurons.
|
| Growth protocol |
Cultures were incubated at 25 C for 5-8 days prior to dissociation and harvesting for Fluorescent Active Cell Sorting (FACS). After this time the cells were fully differentiated into a variety of neuronal. We plated approximately 7.0 x 10E7 cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from FACS sorted fractions using the RNA-STAT 60 kit (Iso Tex Diagnostic, Friendswood TX, USA). The RNA yield was measured by the absorbance at 260 nm and a rough estimate of RNA quality was obtained from the A260/280 ratio. Samples were treated with DNAse I (Ambion, Austin TX, USA) to remove contaminating. RNA clean of any contaminants was monitored by microcapillary electrophoresis (Bioanalyzer 2100, Agilent Technologies, Palo Alto, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
embryo culture 5-7 days, 2.1 kb Gad1(s)-RFP
E_C_Gad_sample3 (H+)
|
| Data processing |
The data presented here have been processed using the R-Project Bioconductor statistical tools package using the affy library. The following were applied: RMA background correction, pmonly probe-level correction, quantile normalization, median-polish summary method. Raw data is provided in the form of .CEL files.
|
| |
|
| Submission date |
Aug 18, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Paul M. Salvaterra |
| E-mail(s) |
[email protected]
|
| Phone |
(626) 301-8364
|
| Fax |
(626) 301-8908
|
| Organization name |
Beckman Research Institute. City of Hope National Medical Center
|
| Department |
Neuroscience
|
| Street address |
1500 E. Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE10773 |
Expression profiling of transgenic Drosophila: neurons |
|
| Relations |
| Reanalyzed by |
GSE119084 |
