|
| Status |
Public on Aug 01, 2014 |
| Title |
DC-SIGN+ LPS rep3 |
| Sample type |
RNA |
| |
|
| Source name |
skin draining LN
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 8-12 weeks
injected with: 5ug of LPS IV
cell subtype: Dendritic cells (DCs)
|
| Growth protocol |
C57BL/6 mice were injected for 12-14 days with human recombinant Flt3L (Celldex), skin draining LNs (inguinal , axillary, brachial, popliteal and cervical were pooled and DC were preapred by standard collagenase D isolation technique. For some samples mice were treated with 5ug of LPS IV and skin draining LN were harvested 24 hours later. Peritoneal macrophages were isolated from untreated mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Purity analysis was performed by nanodrop and Eukaryote Total RNA Pico Series II (Agilent)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified and hybridized on the Illumina MouseRef-8 v2.0 Expression BeadChip
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 16
24 hours after intravenous injection of 5 ug of LPS
|
| Data processing |
The data were normalized using quantile normalization in Genespring v12.5 (Agilent).
|
| |
|
| Submission date |
Dec 23, 2013 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Niroshana Anandasabapathy |
| Organization name |
Brigham and Women’s Hospital
|
| Department |
Department of Dermatology
|
| Street address |
221 Longwood Ave. EBRC 513
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE53588 |
Analysis of classical and migratory DC from the skin draining LNs of Flt3L treated mice and comparison to LPS treated DC from skin draining LN and peritoneal macrophages |
|
