|
| Status |
Public on Dec 28, 2013 |
| Title |
anterior neural fold, stage 18, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Xenopus laevis anterior neural fold, stage 18
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: anterior neural fold
Stage: 18
|
| Treatment protocol |
Neural border, neural plate, anterior neural fold, and animal cap explants were microdissected and incubated with trizol on ice for 5 minutes.
|
| Growth protocol |
Xenopus laevis embryos were grown at 15°C in 1/3 MMR until the desired stage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the standard trizol protocol and eppendorf phase-lock gel tubes, followed by proteinase K digestion, and Qiagen Rneasy columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Hybridization was completed using the standard affymetrix protocol
|
| Scan protocol |
Scanning was completed using an Affymetrix Genechip scanner 3000 7G
|
| Description |
ANF18.14
|
| Data processing |
The data was analysed with R (v.2.14.2) and the Bioconductor Biobase (v.2.14.0) affy library (v.1.32.1 package) with default settings.
|
| |
|
| Submission date |
Dec 27, 2013 |
| Last update date |
Dec 28, 2013 |
| Contact name |
Anne-Helene Monsoro-Burq |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut Curie
|
| Department |
Université Paris Sud
|
| Lab |
U1021 - UMR3347
|
| Street address |
Bat 110
|
| City |
Orsay |
| ZIP/Postal code |
91405 |
| Country |
France |
| |
|
| Platform ID |
GPL1318 |
| Series (2) |
| GSE53678 |
Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers [Xenopus_laevis] |
| GSE53679 |
Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers |
|
