|
| Status |
Public on Nov 17, 2014 |
| Title |
WT liver, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type mouse liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: liver
genotype/variation: wild type
diet: high-fat diet fed starting at 3 months of age for eight weeks.
|
| Treatment protocol |
Liver tissues were harvested under random fed condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the Affymetrix GeneChip 3' IVT Express kit protocol from 250 ng total RNA (Affymetrix GeneChip 3' IVT Express kit Instruction Manual, P/N 702646 Rev. 5).
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on MG-430 PM Strip Arrays using the Affymetrix Gene Atlas system (software version 1.0.4.267).
|
| Scan protocol |
Strip Arrays were scanned using the Affymetrix Gene Atlas system.
|
| Description |
Gene expression data from wild type liver, pool 3
Wild type and Neuregulin 4 (Nrg4) knockout mice were fed high-fat diet starting at 3 months of age for eight weeks.
|
| Data processing |
Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment version 2.15.1. RMA was used to normalize the data and fit log2 transformed expression values.
|
| |
|
| Submission date |
Jan 07, 2014 |
| Last update date |
Nov 17, 2014 |
| Contact name |
Jiandie Lin |
| E-mail(s) |
[email protected]
|
| Phone |
734-615-3512
|
| Organization name |
University of Michigan
|
| Department |
Life Sciences Institute
|
| Street address |
210 Washtenaw Avenue
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE53877 |
Wild type and Neuregulin 4 (Nrg4) knockout mouse livers |
|
