|
| Status |
Public on Dec 31, 2014 |
| Title |
wt, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
wing discs, third larva stage, WT
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain/background: w1118
genotype/variation: WT
tissue: wing imaginal discs
developmental stage: third instar larva
|
| Treatment protocol |
Wing imaginal discs were dissected from 3rd instar larvae (96hr AED) in PBS before being placed in Trizol for RNA extraction.
|
| Growth protocol |
Fly stocks were grown at 25oC on standard media, 60% humidity and a 12 hour light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45oC on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using a GeneChip Scanner 3000 (Affymetrix).
|
| Data processing |
CEL files were generated from DAT files using GCOS software (Affymetrix). Data were processed with Expression Console (Affymetrix) using the PLIER algorithm and Array Assist Lite software was used to generate GC-RMA files (log2 transformed) for each chip.
|
| |
|
| Submission date |
Jan 07, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Julie Secombe |
| E-mail(s) |
[email protected]
|
| Organization name |
Albert einstein college of medicne
|
| Department |
Genetics
|
| Lab |
Julie secombe
|
| Street address |
1300 Morris park ave. Ullmann 809
|
| City |
Bronx |
| State/province |
United states |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE53881 |
The gene expression changes associated with loss of KDM5 (lid) in Drosophila wing discs |
|
