|
| Status |
Public on Sep 19, 2006 |
| Title |
wild_type_embryonic_stem cells_biological_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus embryonic stem cells derived from wild type embryos
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: wild type
not differentiated
|
| Treatment protocol |
no treatment
|
| Growth protocol |
Dulbecco’s modified Eagle’s medium - high glucose supplemented with antibiotics, 15% v/v fetal calf serum and either 103 U/ml of leukemia inhibitory factor (LIF) on gelatinized culture dishes or without LIF on feeder cell layers
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from wild type mouse embryonic stem cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Aug 23, 2006 |
| Last update date |
Sep 19, 2006 |
| Contact name |
Gordin Zupkovitz |
| Organization name |
MFPL
|
| Department |
Medical Biochemistry
|
| Lab |
Seiser
|
| Street address |
Dr. Bohrgasse 9
|
| City |
Vienna |
| ZIP/Postal code |
A-1030 |
| Country |
Austria |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5583 |
Expression data from wild type versus HDAC knock out mouse embryonic stem cells |
|
