|
| Status |
Public on May 02, 2014 |
| Title |
MSKCC_LTS_202 |
| Sample type |
RNA |
| |
|
| Source name |
Glioblastoma, primary tumor
|
| Organism |
Homo sapiens |
| Characteristics |
who grade: IV glioma
survival (months): 50.60
subtype: Proneural
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Lipid Tissue Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Expression analysis of tumors was performed using the Affymetrix U133 2.0 microarray (Affymetrix). Processing of the arrays was per the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Affymetrix U133 2.0 microarray (Affymetrix) protocol.
|
| Scan protocol |
Affymetrix U133 2.0 microarray (Affymetrix) protocol.
|
| Data processing |
The raw CEL files of the 7 MSKCC LTS were imported into Partek without normalization or background correction. The TCGA level 1 data was imported from https://tcga-data.nci.nih.gov/docs/publications/gbm_2013/ (date of download 12/2014) without normalization or background correction. The two datasets were combined (they contained an equal number of probes) and quantile normalization was performed. The values in the matrix file are the normalized values for the MSKCC samples.
|
| |
|
| Submission date |
Jan 14, 2014 |
| Last update date |
May 02, 2014 |
| Contact name |
Naamit Kurshan Gerber |
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE54077 |
Transcriptomal Diversity of Long-Term Glioblastoma Survivors |
|
