|
| Status |
Public on Jan 05, 2015 |
| Title |
RNASeq_htz1-del_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Saccharomyces cerevisiae grown in SD medium
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: MATalpha can1-delta::STE2pr-SP_his5 lyp1-delta his3-delta-1 ura3-delta-0 met15-delta-0 htz1-delta::HpH
|
| Treatment protocol |
none
|
| Growth protocol |
Yeast cells were grown in SD medium to mid-log phase before harvesting for ChIP or RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the Ribopure Purification Kit (Life Technologies) and contaminating genomic DNA was removed by DNaseI digestion. Illumina TruSeq stranded mRNA sample preparation kits were used for library preparation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
Strand-specific mRNA-seq of htz1Δ, replicate 1
|
| Data processing |
Sequence reads were mapped to the S. cerevisiae genome assembly sacCer1 using Bowtie version 0.12.9, allowing up to 2 mismatches and no ambiguously mapped reads.Mapped reads were then converted to WIG files.
Genome_build: sacCer1
Supplementary_files_format_and_content: BedGraph (WIG)
|
| |
|
| Submission date |
Jan 15, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Muxin Gu |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
01223767800
|
| Organization name |
University of Cambridge
|
| Department |
Haematology
|
| Lab |
George Vassiliou
|
| Street address |
Jeffrey Cheah Biomedical Centre, Puddicombe Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13272 |
| Series (1) |
| GSE54105 |
H2A.Z marks antisense promoters and has positive effects on antisense transcript levels in budding yeast |
|
| Relations |
| BioSample |
SAMN02585016 |
| SRA |
SRX433142 |
