Total RNA with small RNAs was extracted with QIAGEN miRNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Extracted molecule
total RNA
Extraction protocol
No specific treatment was performed as the study group included blood samples collected from control and PCOS subjects.
Label
FAM
Label protocol
FAM
Hybridization protocol
n/a
Scan protocol
n/a
Description
N5
Data processing
RNA samples from 8 subjects, average delta CT from technical triplicates reported in data table. Standard Taqman Assay: Quantitative real-time PCR was performed using Applied Biosystems 7900HT system and universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles), FAM channel was measured.
mRNA/miRNA profiling of peripheral blood samples in PCOS patients
Data table header descriptions
ID_REF
VALUE
Data for table was processed using SDS 2.3 software (Applied Biosystems, Foster City, CA) and importing files to RQ (relative quantification) Manager 2.0 software (Applied Biosystems). Normalized by subtracting RNU44-CT to get Delta CT. Average Delta CT from technical triplicates is reported in data table, raw data (*.txt, *.sds) supplied as supplementary files (*.sds readable in SDS and RQ manager software).
