|
| Status |
Public on Jan 29, 2014 |
| Title |
KG1a_Untreated |
| Sample type |
RNA |
| |
|
| Source name |
KG1a, untreated
|
| Organism |
Homo sapiens |
| Characteristics |
tissue of origin: AML-blast
gender: Male
age: 59 years
source: ATCC
cell line: KG1a
treatment: untreated
|
| Treatment protocol |
Cells were left untreated or treated with PMA (phorbol 12-myristate 13-acetate) at 10 ng/ml for 2 hours
|
| Growth protocol |
Cells were maintained in complete media (Isocove's Modified Dulbecco's Media + 100 U/ml penicillin/streptomycin + 100 mM L-Glutamine + 20% fetal calf serum)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Material was isolated using RNeasy mini-columns (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Carried out by Expression Analysis (Durham, NC, USA) following standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Carried out by Expression Analysis (Durham, NC, USA) following standard Affymetrix protocols.
|
| Scan protocol |
Carried out by Expression Analysis (Durham, NC, USA) following standard Affymetrix protocols.
|
| Description |
Gene expression data from unstimulated KG1a
|
| Data processing |
The data were analyzed with Affymetrix GeneChip Operating Software (GCOS1.x) by Expression Analysis
|
| |
|
| Submission date |
Jan 28, 2014 |
| Last update date |
Jan 29, 2014 |
| Contact name |
Gregory B Lesinski |
| Organization name |
Winship Cancer Institute of Emory University
|
| Department |
Hematology and Medical Oncology
|
| Lab |
Lesinski Lab
|
| Street address |
1365C Clifton Rd. NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30324 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE54484 |
Identification of protein kinase C beta 2 regulated genes early in dendritic cell differentiation |
|
