|
| Status |
Public on Feb 04, 2014 |
| Title |
PAXgene whole blood, sepsis_nonsurvivor, Day_3, ID=22 |
| Sample type |
RNA |
| |
|
| Source name |
PAXgene whole blood, sepsis_nonsurvivor, Day 3, ID=22
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
disease status: sepsis nonsurvivor
group_day: NS_D3
group_id: NS_22
gender: M
age (years): 80
severity (apacheii): 20
neutrophil proportion: 0.902061856
site of infection: blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using PAXgene blood RNA kit v2 (PreAnalytix).
|
| Label |
Cy3
|
| Label protocol |
cRNA prepared using Ambion Illumina TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Hybridization protocol as per the Illumina Whole-Genome Gene Expression Direct Hybridization Experienced User Guide.
|
| Scan protocol |
Scanned using default parameters on Illumina BeadArray Reader.
|
| Description |
4674281009_E
"Day 1" refers to the first 24 hrs of ICU admission for the sepsis_survivor and sepsis_nonsurvivor groups, and the day of the first sample collection in healthy controls.
|
| Data processing |
Data pre-processed in GenomeStudio. A detection p value of <0.005 was required in at least 1 sample for probe to be retained. Probes which passed this filter were uploaded into BRB-ArrayTools.
|
| |
|
| Submission date |
Jan 29, 2014 |
| Last update date |
Feb 04, 2014 |
| Contact name |
Grant Parnell |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Sydney
|
| Department |
Nepean Clinical School
|
| Lab |
Intensive Care
|
| Street address |
Nepean Hospital
|
| City |
Kingswood |
| State/province |
NSW |
| ZIP/Postal code |
2747 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE54514 |
Whole blood transcriptome of survivors and nonsurvivors of sepsis |
|
