|
| Status |
Public on Dec 01, 2015 |
| Title |
oocyte samples collected from female 2 sampled in Ria de Aveiro (good quality) |
| Sample type |
RNA |
| |
|
| Source name |
oocytes of R.decussatus
|
| Organism |
Ruditapes decussatus |
| Characteristics |
tissue: oocytes
d-larval rate (%): 88
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Extract-all (Eurobio) procedure.
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent Low input One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 2 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Clam (Ruditapes decussatus) spawned between April 2013 and June 2013 in the experimental bivalve hatchery in Portugal
Gonads RNA of Ruditapes decussatus
|
| Data processing |
Quantile normalization always outperformed cyclic loess and quantile-normalized data were further adjusted for the known between-experiments batch effects by implementing the parametric Combat in R (Johnson et al., 2007).
|
| |
|
| Submission date |
Feb 12, 2014 |
| Last update date |
Dec 01, 2015 |
| Contact name |
Massimo Milan |
| E-mail(s) |
[email protected]
|
| Phone |
+39 0498272941
|
| Organization name |
University of Padova
|
| Department |
Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
|
| Street address |
Viale dell'Università 16
|
| City |
Legnaro |
| State/province |
PD |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL18284 |
| Series (1) |
| GSE54954 |
A microarray-based analysis of oocytes quality of the European clam Ruditapes decussatus |
|
