|
| Status |
Public on Feb 14, 2014 |
| Title |
Liver_Wildtype_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Wildtype mouse, liver
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Wildtype
tissue: Liver
genetic background: C57
Sex: Female
|
| Treatment protocol |
Liver tissues were flash frozen at the time of dissection and stored at -80 C
|
| Growth protocol |
Mice were maintained in the designated animal care facility at the Baylor College of Medicine according to the institutional guideline for the care and use of laboratory animals. SRC-2-/- and wild-type mice were ovariectomized at 6 wk of age. Two weeks later, the mice were killed by cervical dislocation after placing the mice under anesthesia, Avertin (2,2-tribromoethyl alcohol; Sigma-Aldrich,St. Louis, MO).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the QIAGEN RNeasy total RNA isolation kit and transcribed to cDNA
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Data processing |
Normalized expression of probe level data was calculated using the Robust Multiarray Average function (RMA) implemented in Bioconductor's affy-package
|
| |
|
| Submission date |
Feb 13, 2014 |
| Last update date |
Feb 14, 2014 |
| Contact name |
Jae-Wook Jeong |
| Organization name |
Michigan State University
|
| Department |
Department of Obstetrics, Gynecology and Reproductive Biology
|
| Street address |
965 Fee Road
|
| City |
East Lansing |
| State/province |
Michigan |
| ZIP/Postal code |
48824-1316 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE55002 |
Transcription array by profiling in WT and SRC-2 null mouse liver |
|
