|
| Status |
Public on Sep 02, 2006 |
| Title |
Scc1 distribution in scc2-4 cells on chromosome VI (in G2/Nocodazole) |
| Sample type |
genomic |
| |
|
| Source name |
Chromatin Immunoprecipitated DNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain genotype: scc2-4 SCC1-Pk9 GAL-SCC1-HA3
|
| Extracted molecule |
genomic DNA |
| Label |
biotin-11-ddATP
|
| |
|
| Hybridization protocol |
Hybridization, blocking and washing were carried out as previously described (Katou et al., 2003, Nature) (Lengronne et al., 2004, Nature). Samples were hybridized for 16 hours at 42ºC in a hybridization oven (GeneChip Hybridization Oven 640, Affymetrix).
|
| Scan protocol |
Washing and scanning protocol provided by Affymetrix was performed automatically on a fluidics station (GeneChip fluidics station 450, Affymetrix).
|
| Description |
Distribution of Scc1-HA3 (expressed in HU from the Gal promoter in cells at 37°C)on chromosome VI in scc2-4 cells arrested in nocodazole at 37°C.)
|
| Data processing |
Data were processed following the protocol provided by Affymetrix (GCOS ver 1.4)
|
| |
|
| Submission date |
Sep 01, 2006 |
| Last update date |
Sep 01, 2006 |
| Contact name |
Armelle Lengronne |
| E-mail(s) |
[email protected]
|
| Phone |
+33 499 61 9955
|
| Organization name |
CNRS
|
| Department |
Institut de Genetique Humaine
|
| Lab |
Equipe Pasero
|
| Street address |
141 rue de la Cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34396 |
| Country |
France |
| |
|
| Platform ID |
GPL347 |
| Series (1) |
| GSE5719 |
Cohesin and cohesion establishment factors distribution on S.Cerevisiae chromosome VI during the cell cycle |
|
