|
| Status |
Public on May 14, 2014 |
| Title |
Hif-1α+/+ |
| Sample type |
RNA |
| |
|
| Source name |
Colon
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6 to 8 weeks old
genotype/variation: Hif-1α+/+
tissue: Colon mucosal scrapings
|
| Treatment protocol |
Colon mucosal scrapings were got from 6 to 8 weeks old mice
|
| Growth protocol |
All mice were C57BL/6 background and maintained in standard cages in a light and temperature-controlled room and were allowed standard chow and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Ambion WT kit protocol from 250 ng total RNA (GeneAtlas™ WT Expression Kit User Manual P/N 702935 Rev. 3).
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.7 ug of cDNA were hybridized for 20 hr at 48C on Mouse Gene 1.1 ST Array Strips using the Affymetrix Gene Atlas system (software version 1.0.4.267).
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system.
|
| Description |
Gene expression data from colons.
Shah_019
|
| Data processing |
Data was analyzed using the oligo Bioconductor package implemented in the R-statistical environment. RMA was used to fit log2 transformed expression values.
|
| |
|
| Submission date |
Mar 14, 2014 |
| Last update date |
May 14, 2014 |
| Contact name |
XIANG XUE |
| E-mail(s) |
[email protected]
|
| Phone |
7346155041
|
| Organization name |
Umich
|
| Street address |
1301 E. Catherine Street
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE55915 |
Gene Expression profiles of colon from Hif-1α+/+, Hif-1αLSL/LSL |
|
