|
| Status |
Public on Mar 18, 2014 |
| Title |
Prostate Cancer_MS 36D8 |
| Sample type |
RNA |
| |
|
| Source name |
malignant tissue_TMPRSS2:ERG fusion negative
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Malignant prostate tissue
tmprss2: ERG fusion status: negative
|
| Treatment protocol |
Benign or PCa tissues were homogenized using a TissueLyser (Qiagen) at 28 Hz for 5 min.
|
| Growth protocol |
A biopsy punch was used to select the PCa tissues from the OCT prostate sample blocks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol reagent. RNA was quantified by NanoDrop ND-1000 spectrophotometer, and quality was evaluated with Agilent RNA 6000 NanoChip and the 2100 Bioanalyzer, with 28S/18S ratios and RIN determined by 2100 Expert software.
|
| Label |
biotin
|
| Label protocol |
Biotin-UTP was incorporated during the overnight in vitro transcription step according to the manufacturer's (Ambion® | Life Technologies) protocol.
|
| |
|
| Hybridization protocol |
cRNA (15 μg) was fragmented and hybridized to arrays according to the manufacturer's (Ambion® | Life Technologies) protocols.
|
| Scan protocol |
GeneArray Scanner G2500A (Hewlett-Packard)
|
| Description |
MS 36D8
|
| Data processing |
BioConductor R packages
|
| |
|
| Submission date |
Mar 17, 2014 |
| Last update date |
Mar 18, 2014 |
| Contact name |
M. Simo Arredouani |
| E-mail(s) |
[email protected]
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Department |
Surgery
|
| Street address |
330 Brookline Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE55945 |
Gene Expression Profiling of Prostate Benign and Malignant Tissue |
|
