|
| Status |
Public on Apr 12, 2014 |
| Title |
human CD19+ IgD+ CD27- cells from tonsil rep2 |
| Sample type |
RNA |
| |
|
| Source name |
human CD19+ IgD+ CD27- cells from tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tonsil
cell type: naive B cells (CD19+ IgD+ CD27-)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated by Trizol reagent (Invitrogen, Carlsbad, CA, USA). RNA quality was checked using total RNA (small fraction chip) with the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA) and by 1% agarose electrophoresis, following the standard procedure.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
100 ng of total RNA were hybridized on an Agilent Human miRNA one-color microarray, following the manufacturer’s instructions (Agilent Technologies)
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
|
| Description |
naive 2
|
| Data processing |
Between-array median normalization was carried out to render miRNA expression datasets comparable using Genespring GX9 software.
|
| |
|
| Submission date |
Mar 26, 2014 |
| Last update date |
Apr 12, 2014 |
| Contact name |
Lorena Di Lisio |
| E-mail(s) |
[email protected]
|
| Organization name |
CNIO
|
| Street address |
C/ Melchor Fernández Almagro, 3
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9081 |
| Series (1) |
| GSE29493 |
microRNA signature in b-cell lymphomas and control human B cells from fresh tonsils |
|
