|
Status |
Public on Apr 30, 2014 |
Title |
400.75 |
Sample type |
SRA |
|
|
Source name |
polyp
|
Organism |
Acropora aff. hyacinthus sp. E JTL-2012 |
Characteristics |
pool of origin: 300 pool transplanted into: 400
|
Treatment protocol |
All colonies were sampled within 10 minutes of noon on August 30, 2011
|
Extracted molecule |
total RNA |
Extraction protocol |
Ambion RNAqueous Kit Illumina TruSeq kits, Illumina barcoded: 12 per lane
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
transplanted Aug 3, 2010. Sampled in situ Aug 30, 2011
|
Data processing |
Mapped using bwa (fraction missing alignments = 0.005) assembly as in Barshis et al. 2013 Normalization was performed with DESeq using default parameters filtered so mean # mapped reads>5 Genome_build: de novo built assembly described in Barshis et al. 2013 Supplementary_files_format_and_content: Text file containing normalized mapped read counts
|
|
|
Submission date |
Mar 27, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Stephen Palumbi |
E-mail(s) |
[email protected]
|
Phone |
831-655-6214
|
Organization name |
Stanford University
|
Department |
Biology
|
Lab |
Hopkins Marine Station
|
Street address |
120 Ocean View Blvd
|
City |
Pacific Grove |
State/province |
CA |
ZIP/Postal code |
93950 |
Country |
USA |
|
|
Platform ID |
GPL18475 |
Series (1) |
GSE56278 |
Mechanisms of coral response to climate change |
|
Relations |
BioSample |
SAMN02709459 |
SRA |
SRX501942 |