Female, 22 year old doter donate a small bowel to her mother 45 year old, the biopsy from the transplanted intestine 6 weeks post transplant, with signs of rejection
Extracted molecule
total RNA
Extraction protocol
RNeasy micro kit Qiagen, Valencia, CA, USA Cat No 75142 according to the manufacturer's instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard BioArray RNA IVT labeling kit Enzo Life Science, Farmingdale, NY, USA
Hybridization protocol
Following fragmentation, 10 microg of cRNA were hybridized on 45 C on Affymetrix HG-U133_Plus_2 human GeneChip, GeneChips were washed and stained in the Affymetrix Fluidics according to their protocol, EukGE-WS2v4_450
Scan protocol
GeneChips were scanned using Pixel Size 1.56, Filter 570, Scanner ID 50208790, Scanner Type M10
Description
Intestine, 6 weeks post transplant under immunosuppressant rep1. Gene expression data from transplanted intestinal mucosa, when there are signs and symptoms of rejection.
Data processing
All microarray data analysis was done using the Genespring 6.1 software package Agilent Technologies, Palo Alto, CA, USA. Gene lists generated, were evaluated for GO biological functions and pathway analysis using David 2.0 NIH, Bethesda, MD, USA and Pathway Assist 3.0 Stratagene, La Jolla, CA,
