|
| Status |
Public on Aug 26, 2009 |
| Title |
253jp |
| Sample type |
RNA |
| |
|
| Source name |
bladder cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
human bladder cancer cell line
|
| Treatment protocol |
Isogenic T24T cells, bladder carcinoma–derived cell lines, primary human bladder carcinomas, and normal bladder tissues were profiled on HG-U133A GeneChipTM arrays (Affymetrix, Santa Clara, CA).
|
| Growth protocol |
For the isogenic T24T cell pair containing vector or RhoGDI2 constructs, duplicate RNA samples were generated from independent cell cultures.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array HG-U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from bladder cancer cell lines before drug treatment.
|
| Data processing |
The data were analyzed with RMAexpress (Robust Microarrya Analysis) using its default analysis settings and quantile normalization method.
|
| |
|
| Submission date |
Sep 15, 2006 |
| Last update date |
Aug 26, 2008 |
| Contact name |
Jae K Lee |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Virginia
|
| Department |
Public Health Sciences
|
| Street address |
PO Box 800717
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE5845 |
Bladder Cancer 40 Cell Lines |
|
