|
| Status |
Public on Apr 05, 2014 |
| Title |
CS_EDTA_antibiotic |
| Sample type |
RNA |
| |
|
| Source name |
CS antibiotic + EDTA at 0.07g/L
|
| Organism |
Burkholderia thailandensis E264 |
| Characteristics |
medium: Calf serum
temp °c: 37
ID: 50
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total bacterial RNA for each experimental condition was extracted using the TRIzol method.
|
| Label |
biotin
|
| Label protocol |
Microarray experiments were performed by the Genomics and Microarray Core at the University of Colorado, Denver in 2009.
|
| |
|
| Hybridization protocol |
Hybridization followed standard Affymetrix procedures.
|
| Scan protocol |
Arrays were scanned using an Affymetrix GeneChip scanner.
|
| Description |
carbenicillin (100 μg/ml), chloramphenicol (30 μg/ml), erythromycin (200 μg/ml, and kanamycin (50 μg/ml)
|
| Data processing |
For each time course experiment, the samples were processed and normalized using RMA in the R affy package.
|
| |
|
| Submission date |
Apr 03, 2014 |
| Last update date |
Apr 05, 2014 |
| Contact name |
Chris J Stubben |
| Organization name |
Los Alamos National Lab
|
| Department |
Bioscience Division
|
| Street address |
MS M888
|
| City |
Los Alamos |
| State/province |
NM |
| ZIP/Postal code |
87544 |
| Country |
USA |
| |
|
| Platform ID |
GPL18529 |
| Series (1) |
| GSE56502 |
Differential expression of small RNAs from Burkholderia thailandensis in response to varying environmental and stress conditions |
|
