|
| Status |
Public on Dec 12, 2014 |
| Title |
MS25_Sample3_IL1_beta_5.6_20080819 |
| Sample type |
RNA |
| |
|
| Source name |
human blood monocyte stimulated with IL1b for 4h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human blood monocyte
treatment: IL1b
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with chaotropic ions and silica membran according to the manufacturer's instructions (Macherey-Nagel RNA isolation user manual).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Affymetrix Gene Chip Expression 3´Amplification: one-cycle cDNA synthesis and IVT labeling; Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
hybridization to Affymetrix (Santa Clara, CA) Human Genome 133 Plus 2.0 Gene Chip arrays
|
| Scan protocol |
Fluorescent signals were detected by the Hewlett-Packard G2500A Gene Array Scanner (Affymetrix).
|
| Data processing |
Data were imported into Partek Genomics Suite V6.6 (PGS) (Partek) using RMA (Robust Multi-array Average, an algorithm used for background correction, log2-transformation and quantile normalization of Affymetrix expression data) prior to batch-correction.
|
| |
|
| Submission date |
Apr 10, 2014 |
| Last update date |
Dec 14, 2014 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
[email protected]
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE56681 |
Genome-wide expression analysis demonstrates a dominant role of TLR4 for activation of human phagocytes by the alarmin MRP8 |
|
