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| Status |
Public on Apr 22, 2014 |
| Title |
MEFs_SKO+Flag_rep1 |
| Sample type |
RNA |
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| Source name |
MEF, SKO+Flag, Day8, Rep1
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| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic fibroblast
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| Treatment protocol |
MEFs were infected with certain virus and then cultured in DMEM/High Glucose plus +15%FBS+NEAA+Gluta Max+Sodium Pyruvate+lif for 6 days.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using mirVana™ miRNA Isolation Kit (Cat#AM1560, Ambion, Austin, TX, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100(Agilent technologies, Santa Clara, CA, US)
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| Label |
Cy3
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| Label protocol |
miRNA molecular in total RNA was labeled by miRNA Complete Labeling and Hyb Kit(Cat#5190-0456, Agilent technologies, Santa Clara, CA, US)followed the manufacturer’s instructions, labeling section.
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| Hybridization protocol |
Each slide was hybridized with 100ng Cy3-labeled RNA using miRNA Complete Labeling and Hyb Kit(Cat#5190-0456, Agilent technologies, Santa Clara, CA, US)in hybridization Oven(Cat#G2545A, Agilent technologies, Santa Clara, CA, US)at 55℃，20rpm for 20 hours according to the manufacturer’s instructions, hybridization section. After hybridization, slides were washed in staining dishes (Cat#121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat#5188-5327, Agilent technologies, Santa Clara, CA, US).
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| Scan protocol |
Slides were scanned by Agilent Microarray Scanner(Cat#G2565BA，Agilent technologies, Santa Clara, CA, US)and Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US)with default settings.
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| Description |
miRNAs expression of MEFs infected with SKO+Flag
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| Data processing |
Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0(Agilent technologies, Santa Clara, CA, US).
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| Submission date |
Apr 21, 2014 |
| Last update date |
Apr 22, 2014 |
| Contact name |
Keshi Chen |
| Organization name |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Science
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| Street address |
190 Kai Yuan Avenue
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| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platform ID |
GPL17912 |
| Series (2) |
| GSE56942 |
miRNAs regulated by Gadd45a during somatic cell reprogramming |
| GSE56944 |
Genes and miRNAs regulated by Gadd45a during somatic cell reprogramming |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSM1372017_SKO_253118410272_S01_miRNA_107_Sep09_105_1_3.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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