|
| Status |
Public on May 02, 2014 |
| Title |
Ezh2_Delete_2day_3 |
| Sample type |
RNA |
| |
|
| Source name |
Differentiated NSCs
|
| Organism |
Mus musculus |
| Characteristics |
age: P7-P9
tissue: SVZ
|
| Treatment protocol |
Ezh2 was acutely deleted by the addition of 4-hydroxytamoxifen (4OHT) to SVZ NSC cultures generated from UBC-CreERT2;Ezh2F/F from P3-4
|
| Growth protocol |
SVZ NSCs were passaged 5 times in proliferation medium(DMEM/F12/N2 – Invitrogen, 5% FCS - Hyclone, 20 ng ml-1 EGF, 20 ng ml-1 bFGF, 35 μg ml-1 bovine pituitary extract – Peprotech) before switching to differentiation media (same as proliferation media except with 2% FCS and no EGF or bFGF) for the specified time.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol (Life Technologies), purified using the RNeasy Plus Mini Kit (Qiagen) and quantified using the NanoDrop spectrophotometer. Samples were checked for quality using an Agilent TapeStation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was generated from total RNA using an Ambion Illumina RNA amplification kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 23, 2014 |
| Last update date |
May 02, 2014 |
| Contact name |
William Hwang |
| Organization name |
UCSF
|
| Department |
Neurosurgery
|
| Lab |
Lim
|
| Street address |
Lim Lab - Box 0525, 35 Medical Center Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE56988 |
Timecourse analysis of Ezh2-dependent gene expression by SVZ NSCs during differentiation |
|
