|
| Status |
Public on Apr 29, 2014 |
| Title |
WT zinc X zur zinc - rep4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
zur mutant zinc
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype/variation: zur mutant
cultured with: 200 µM ZnCl2 for 1 h
|
| Treatment protocol |
The culture was supplemented with 200 µM ZnCl2 for 1 h.
|
| Growth protocol |
Cultures were grown until midlog growing phase in M2 medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Standard Cy3-dCTP and Cy5-dCTP incorporations
|
| |
|
| Channel 2 |
| Source name |
WT zinc
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype/variation: wild type
cultured with: 200 µM ZnCl2 for 1 h
|
| Treatment protocol |
The culture was supplemented with 200 µM ZnCl2 for 1 h.
|
| Growth protocol |
Cultures were grown until midlog growing phase in M2 medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Standard Cy3-dCTP and Cy5-dCTP incorporations
|
| |
|
| |
| Hybridization protocol |
Standard Agilent protocol Agilent protocol
|
| Scan protocol |
Standard Agilent protocol Agilent protocol
|
| Description |
RNA after 2 hours of addition of 200 micromolars of zinc
|
| Data processing |
Data were extracted and normalized using Agilent Feature Extraction Software 9.0. Agilent output files were then opened using Excel and the log10 values extracted with no additional processing or filterin
|
| |
|
| Submission date |
Apr 28, 2014 |
| Last update date |
Apr 29, 2014 |
| Contact name |
Ricardo Ruiz Mazzon |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Biomedical Sciences from University of São Paulo
|
| Department |
Microbiology
|
| Lab |
222
|
| Street address |
Avenida Professor Lineu Prestes, 1374
|
| City |
São Paulo |
| State/province |
SP |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL10469 |
| Series (1) |
| GSE57136 |
Analysis of the Caulobacter crescentus Zur regulon reveals novel insights in zinc acquisition by TonB-dependent outer membrane proteins |
|
