|
| Status |
Public on May 30, 2014 |
| Title |
Sample 4 |
| Sample type |
genomic |
| |
|
| Source name |
Blood culture
|
| Organism |
Staphylococcus aureus |
| Characteristics |
gram status: Gram-positive
culture system: BACTEC
treatment: Supernatant
source: Colony diluent
|
| Treatment protocol |
Direct testing of culture broth (Standard) or after centrifugation of culture broth (Supernatant)
|
| Growth protocol |
Blood culture bottle
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Automatic extractor in Verigene system of genomic DNA
|
| Label |
Silver-enhanced gold nanoparticle
|
| Label protocol |
Silver-enhancement of gold nanoparticle probe signals (light scatter).
|
| |
|
| Hybridization protocol |
Automatic Verigene instrument. Gold nanoparticles are functionalized with target DNA-specific oligonucleotide probes and mixed with genomic DNA from samples. Gold nanoparticle-targetDNA duplexes hybridize with target-specific capture oligonucleotides, each in a separate spot on the microarray. Gold nanoparticles are coated with silver to amplify signal.
|
| Scan protocol |
Verigene Reader used to scan microarray. Signal strength on the spots is proportional to the concentration of target DNA in the sample.
|
| Description |
SAMPLE_004
|
| Data processing |
Scatter signal intensity after illumination at 150 ms exposure without normalization, without transformation. The non-normalized signals form the basis of conclusions in the accompanying manuscript.
|
| |
|
| Submission date |
May 02, 2014 |
| Last update date |
May 30, 2014 |
| Contact name |
Seon Young Kim |
| Organization name |
Seoul National University College of Medicine
|
| Street address |
101 Daehak-ro, Jongno-gu,
|
| City |
Seoul |
| ZIP/Postal code |
110-744 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL18634 |
| Series (1) |
| GSE57245 |
Identification of Gram-positive bacteria using BC-GP assay |
|
