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Sample GSM1377738 Query DataSets for GSM1377738
Status Public on May 30, 2014
Title Sample 5
Sample type genomic
 
Source name Blood culture
Organism Staphylococcus aureus
Characteristics gram status: Gram-positive
culture system: BACTEC
treatment: Supernatant
source: Colony diluent
Treatment protocol Direct testing of culture broth (Standard) or after centrifugation of culture broth (Supernatant)
Growth protocol Blood culture bottle
Extracted molecule genomic DNA
Extraction protocol Automatic extractor in Verigene system of genomic DNA
Label Silver-enhanced gold nanoparticle
Label protocol Silver-enhancement of gold nanoparticle probe signals (light scatter).
 
Hybridization protocol Automatic Verigene instrument. Gold nanoparticles are functionalized with target DNA-specific oligonucleotide probes and mixed with genomic DNA from samples. Gold nanoparticle-targetDNA duplexes hybridize with target-specific capture oligonucleotides, each in a separate spot on the microarray. Gold nanoparticles are coated with silver to amplify signal.
Scan protocol Verigene Reader used to scan microarray. Signal strength on the spots is proportional to the concentration of target DNA in the sample.
Description SAMPLE_005
Data processing Scatter signal intensity after illumination at 150 ms exposure without normalization, without transformation. The non-normalized signals form the basis of conclusions in the accompanying manuscript.
 
Submission date May 02, 2014
Last update date May 30, 2014
Contact name Seon Young Kim
Organization name Seoul National University College of Medicine
Street address 101 Daehak-ro, Jongno-gu,
City Seoul
ZIP/Postal code 110-744
Country South Korea
 
Platform ID GPL18634
Series (1)
GSE57245 Identification of Gram-positive bacteria using BC-GP assay

Data table header descriptions
ID_REF
VALUE Scatter signal intensity of SPOT without normalization

Data table
ID_REF VALUE
fstasp01 63982
fstasp02 63982
fstasp03 63982
fstasp04 63982
fstasp05 63982
fstasp06 63982
faur01 63982
faur02 63982
faur03 63982
faur04 63982
faur05 63982
faur06 63982
fepi01 49
fepi02 152
fepi03 1482
fepi04 129
fepi05 7861
fepi06 15583
flud01 997
flud02 7984

Total number of rows: 178

Table truncated, full table size 2 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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