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Sample GSM1377804 Query DataSets for GSM1377804
Status Public on May 30, 2014
Title Sample 71
Sample type genomic
 
Source name Blood culture
Organism Enterococcus gallinarum
Characteristics gram status: Gram-positive
culture system: BacTALERT
treatment: Supernatant
source: Colony diluent
Treatment protocol Direct testing of culture broth (Standard) or after centrifugation of culture broth (Supernatant)
Growth protocol Blood culture bottle
Extracted molecule genomic DNA
Extraction protocol Automatic extractor in Verigene system of genomic DNA
Label Silver-enhanced gold nanoparticle
Label protocol Silver-enhancement of gold nanoparticle probe signals (light scatter).
 
Hybridization protocol Automatic Verigene instrument. Gold nanoparticles are functionalized with target DNA-specific oligonucleotide probes and mixed with genomic DNA from samples. Gold nanoparticle-targetDNA duplexes hybridize with target-specific capture oligonucleotides, each in a separate spot on the microarray. Gold nanoparticles are coated with silver to amplify signal.
Scan protocol Verigene Reader used to scan microarray. Signal strength on the spots is proportional to the concentration of target DNA in the sample.
Description SAMPLE_071
Data processing Scatter signal intensity after illumination at 150 ms exposure without normalization, without transformation. The non-normalized signals form the basis of conclusions in the accompanying manuscript.
 
Submission date May 02, 2014
Last update date May 30, 2014
Contact name Seon Young Kim
Organization name Seoul National University College of Medicine
Street address 101 Daehak-ro, Jongno-gu,
City Seoul
ZIP/Postal code 110-744
Country South Korea
 
Platform ID GPL18634
Series (1)
GSE57245 Identification of Gram-positive bacteria using BC-GP assay

Data table header descriptions
ID_REF
VALUE Scatter signal intensity of SPOT without normalization

Data table
ID_REF VALUE
fstasp01 211
fstasp02 842
fstasp03 94
fstasp04 66
fstasp05 86
fstasp06 112
faur01 418
faur02 398
faur03 55
faur04 73
faur05 762
faur06 671
fepi01 24
fepi02 0
fepi03 0
fepi04 24
fepi05 98
fepi06 117
flud01 41
flud02 92

Total number of rows: 178

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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