|
| Status |
Public on Jun 01, 2015 |
| Title |
8h p.i._2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila S2 Invitrogen cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: Drosophila S2 Invitrogen cells
infection: Drosophila C Virus infection (DCV)
time: 8h
|
| Treatment protocol |
S2 cells were seeded in 6-well plates in 2mL of Schneider's media, with 2,5.10^6 cells per well. After media removal, 0.5mL of virus inoculum (Multiplicity of Infection = 10) was added. After one hour incubation, inoculums were replaced with 2mL of fresh media.
|
| Growth protocol |
Drosophila S2 cells (Invitrogen) were cultured at 25°C in Schneider’s Drosophila Media (Gibco) supplemented with 10% heat-inactivated Fetal Calf Serum (PAA), 50 U/mL Penicillin and 50 μg/mL Streptomycin (Gibco)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy column purification followed by on-colomn DNAseI treatment, according to the manufacturer's instructions (Qiagen)
|
| Label |
biotin
|
| Label protocol |
GeneChip WT Terminal Labeling Kit
|
| |
|
| Hybridization protocol |
17 hours at 45 degrees in Affymetrix hybridization oven at 60 rpm,washing and staining in Fluidics station 450 using stain cocktails: SAPE and antibody solution, (protocol FS450_0001)
|
| Scan protocol |
GeneChip scanner 3000 7G
|
| Description |
8hpi 2
|
| Data processing |
RMA Background Correction using Partek software
|
| |
|
| Submission date |
May 08, 2014 |
| Last update date |
Jun 01, 2015 |
| Contact name |
Ronald van Rij |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Radboud Institute for Molecular Life Sciences - Radboud University Medical Center
|
| Street address |
Geert Grooteplein 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE57434 |
Transcriptional response of Drosophila S2 cells in response the Drosophila C Virus infection (DCV) |
|
