|
| Status |
Public on Oct 13, 2006 |
| Title |
atopic eczema P5 |
| Sample type |
RNA |
| |
|
| Source name |
Skinbiopsy from patient with atopic eczema
|
| Organism |
Homo sapiens |
| Characteristics |
Atopic eczema
|
| Treatment protocol |
RNA extracted from frozen biopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
According to the instructions from Affymetrix.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from skin biopsy from healthy control.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 02, 2006 |
| Last update date |
Oct 13, 2006 |
| Contact name |
Mikael Benson |
| E-mail(s) |
[email protected]
|
| Phone |
+46 31 343 5162
|
| Fax |
+46 31 848952
|
| Organization name |
Queen Silvia Children's Hospital
|
| Department |
Paediatrics
|
| Street address |
Smorslottsgatan
|
| City |
Gothenburg |
| ZIP/Postal code |
416 85 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6012 |
Skin biopsies from atopic eczema and healthy controls |
|
