|
| Status |
Public on Aug 01, 2014 |
| Title |
dorsal trunk SKP-derived Schwann cell sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
adult rat dorsal trunk SKP-derived Schwann cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley rat
tissue: dermis
cell type: dorsal trunk SKP-derived Schwann cell
|
| Treatment protocol |
SKPs proliferated as spheres. After one or two passages, spheres were placed in Trizol reagent. Schwann cells or SKP-Schwann cells were passaged once, grown until confluent, then washed and placed in Trizol reagent.
|
| Growth protocol |
Dorsal trunk skin samples were cut into small pieces and enzymatically digested and filtered until single cells were isolated. These cells were cultured in proliferation-promoting conditions as previously described. The resulting SKP spheres were harvested for RNA or plated in Schwann cell growth media. Sciatic nerves were dissected and enzymatically digested to liberate single cells. These were plated and grown in Schwann cell growth media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed with the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Rat Gene 1.0 ST Arrays (Affymetrix) and scanned at the HSC Microarray Facility.
|
| Scan protocol |
The hybridized microarray image was scanned with the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from adult rat dorsal trunk SKP-derived Schwann cells
|
| Data processing |
Raw probe intensity values were background corrected, normalized with quantile normalization, transformed into the log2 scale, and summarized into probesets using the Robust Multichip Analysis (RMA) algorithm at the gene level in the Affymetrix Expression Console program.
|
| |
|
| Submission date |
May 09, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Freda Miller |
| E-mail(s) |
[email protected]
|
| Organization name |
Hospital for Sick Children
|
| Street address |
555 University Avenue
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6247 |
| Series (2) |
| GSE57505 |
Direct genesis of functional rodent and human Schwann cells from skin mesenchymal precursors (rat) |
| GSE57519 |
Direct genesis of functional rodent and human Schwann cells from skin mesenchymal precursors |
|
