|
| Status |
Public on Jul 15, 2014 |
| Title |
Control siRNA at T48, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
AsPC-1 transfected with 20nM control siRNA at 48 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: AsPC-1
treatment: transfected with 20nM Ctrl siRNA, 48h
|
| Treatment protocol |
See growth protocol
|
| Growth protocol |
AsPC-1 cells were grown to 70% confluency in standard growth conditions (RPMI + 10% FCS) and maintained in these conditions for the period (6 or 24 hours) with either 10uM ICG-001 or vehicle (DMSO, V/V). Separately, AsPC1 cells were also transfected with 20 nM control or CTNNB1 siRNA using lipofectamine 2000 with RNA extracted at 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen Rneasy mini kit per manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix standard procol
|
| |
|
| Hybridization protocol |
Affymetrix standard procol
|
| Scan protocol |
Affymetrix standard procol
|
| Description |
Gene expression data for AsPC1 cells transfected with control siRNA for 48 hours
|
| Data processing |
Data analysis was performed in the dChip Analysis software package(31) using invariant set normalization and signal intensities summarized using the MBEI (model-based expression index) algorithm with mismatch probe option for background subtraction. Initial gene filtering criteria was set at >10% present call and variance across samples of 0.4<standard deviation/mean<1000.
|
| |
|
| Submission date |
May 16, 2014 |
| Last update date |
Jul 15, 2014 |
| Contact name |
David Dawson |
| Organization name |
UCLA
|
| Department |
Pathology and Laboratory Medicine
|
| Street address |
10833 LeConte Avenue
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1732 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE57728 |
Expression data from AsPC1 cells treated with ICG-001 |
|
