|
| Status |
Public on Feb 14, 2017 |
| Title |
droso_72hr_2 |
| Sample type |
RNA |
| |
|
| Source name |
KC167 cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
treatment: mms
time period: 72hr
replicate: 2
|
| Treatment protocol |
MMS exposure was 40 µg/mL final concentration in fresh medium.
|
| Growth protocol |
Kc167 cells were grown cells in Schneider's media supplemented with 10% inactivated FBS plus antibiotics in a 22°C humidified incubator. Cells were kept and treated in the exponential phase of growing (60-70% confluence).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using the RNeasy protocol (Qiagen) and the purity of prepared RNA was determined using Agilent 2100 BioAnalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 µg of cRNA were hybridized for 16 hr at 45ºC on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GC300 scanner 7G.
|
| Description |
MMS 72hr-2
|
| Data processing |
Data was generated by using the robust multi-array average (RMA) expression measure implemented in R, directly processed from CEL files
|
| |
|
| Submission date |
May 19, 2014 |
| Last update date |
Jun 27, 2018 |
| Contact name |
Yidong Chen |
| E-mail(s) |
[email protected]
|
| Phone |
2105629163
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Population Health Sciences
|
| Street address |
8403 Floyd Curl Drive, MSC 7784
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE57788 |
MMS induced expression changes (Drosophila) |
| GSE57801 |
MMS induced expression changes |
|
