|
Status |
Public on Oct 13, 2006 |
Title |
A549DCA: DCA treated lung carcinoma cells |
Sample type |
RNA |
|
|
Source name |
DCA treated Cultured Lung carcinoma cells
|
Organism |
Homo sapiens |
Characteristics |
'Organ: Lung', 'disease : Carcinoma', 'Morphology: epithelial', 'Age: 58 years old', 'Sex: Male', 'Ethnicity : Caucasian'
|
Biomaterial provider |
American type culture collection (ATCC) Manassas, VA USA.
|
Treatment protocol |
48 h with dichloroacetate 0.5 mM (Sigma, St Louis)
|
Growth protocol |
The A549 cells were maintained on F12K medium, Media was supplemented with 10% FBS and 5% PSF (Gibco) as antibiotic. Cultures were incubated for up to 48h prior to harvesting.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from A549 cells was extracted using Quiagen RNeasy kit (Ontario, Canada).
|
Label |
Biotin
|
|
|
Description |
Characteristics: Organ: Lung disease : Carcinoma Morphology: epithelial Age: 58 years old Sex: Male Ethnicity : Caucasian
|
Data processing |
MAS5.0
|
|
|
Submission date |
Oct 11, 2006 |
Last update date |
Oct 11, 2006 |
Organization |
Ottawa Hospital Research Institute |
Phone |
(613) 737-8899 -73255
|
Department |
Cellular and Molecular Medicine
|
Lab |
Ottawa Bioinformatics Core Facility
|
Street address |
501 Smyth Rd.
|
City |
Ottawa |
State/province |
ON |
ZIP/Postal code |
K1H 8L6 |
Country |
Canada |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE6014 |
A Mitochondria-K+ Channel Axis Is Suppressed in Cancer & Its Normalization Promotes Apoptosis and Inhibits Cancer Growth |
|