|
| Status |
Public on May 23, 2014 |
| Title |
CSBwt 24h post UV rep_1 |
| Sample type |
RNA |
| |
|
| Source name |
Immortalized human fibroblasts representing trancated CSB (ERCC6) protein and restored by stable transfection of pcDNA3 CSB wt vector
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Dermal fibroblasts
genotype: CSB taged by: HA N-term, His C-term
treatment: UV-C 10 J/m2
|
| Treatment protocol |
UV-C 10 J/m2, alpha-amanitin 10 micrograms/ml
|
| Growth protocol |
Dulbecco/HamF10 medium containing 10% FCS and 40 mg/mL gentamycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
GenElute Mammalian Total RNA Miniprep Kit
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip WT Terminal Labeling
|
| |
|
| Hybridization protocol |
Hybridization User manual for use with the Ambion Expression Kit : Part Number 702808 Rev.1
|
| Scan protocol |
Affymetrix GeneChip Expression Wash, Stain and Scan User Manual for Cartridge Arrays : Part Number 702731 Rev.3
|
| Description |
UV-C 10 J/m2
|
| Data processing |
After chip scanning, a raw image file (.DAT) was generated by Affymetrix Command Console (AGCC v 4.0). After alignment of a grid (.GRD file), a smoothened image file (.CEL) was automatically created. This later file was then used to calculate the signal intensity for each probeset on the chip using RMA algorithms with the Affymetrix Software Expression Console v 1.1
|
| |
|
| Submission date |
May 23, 2014 |
| Last update date |
May 23, 2014 |
| Contact name |
Egly Jean-Marc |
| Organization name |
IGBMC
|
| Street address |
1 rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67400 |
| Country |
France |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE57923 |
Regulatory interplay of Cockayne syndrome B ATPase and stress-response gene ATF3 following genotoxic stress |
|
