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Status
Public on Feb 01, 2007
Title
UCB-MSC_UCB2_rep3A
Sample type
RNA
Source name
human umbilical cord blood-derived mesenchymal stem cell population 2, replicate 3
Organism
Homo sapiens Characteristics
Tissue: umbilical cord blood-derived mesenchymal stem/stromal cell
Biomaterial provider
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103, USA, [email protected] http://www.coriell.org/
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cells in culture using the RNAeasy Mini Kit (Qiagen), according to manufacturer's protocol
Label
Biotin
Label protocol
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
Hybridization protocol
Hybridization was carried out according to Affymetrix protocol.
Description
none
Data processing
RMA method normalization implemented in BioConductor
Submission date
Oct 12, 2006
Last update date
Oct 13, 2006
Contact name
Kenro Kusumi
E-mail(s)
[email protected] Phone
480-727-8993
Fax
480-965-6899
URL
http://sols.asu.edu/faculty/kkusumi.php Organization name
Arizona State University
Department
School of Life Sciences
Street address
PO Box 874501
City
Tempe
State/province
AZ
ZIP/Postal code
85287
Country
USA
Platform ID
GPL96 Series (1)
GSE6029 Cord Blood-Derived Mesenchymal Stem Cells with Distinct Growth Kinetics, Differentiation Potentials, Expression Profiles
Data table header descriptions ID_REF VALUE RMA expression estimate
Data table ID_REF VALUE 1007_s_at
8.65131800504593
1053_at
4.24768060931687
117_at
5.0003545449649
121_at
7.32905325214115
1255_g_at
2.66527395520726
1294_at
5.97023541666336
1316_at
4.57553996836615
1320_at
4.81218102574134
1405_i_at
3.0241749141703
1431_at
2.95008274744615
1438_at
5.42793011389361
1487_at
6.24201461294374
1494_f_at
5.12109519800215
1598_g_at
11.5118282541603
160020_at
9.15594135475008
1729_at
6.562013912712
177_at
4.68278057017295
1773_at
5.71604886235465
179_at
8.06617848516241
1861_at
6.04465532240425
22283 605 Kbytes .Supplementary file Size Download File type/resource GSM139890.CEL.gz
3.1 Mb
(ftp) (http) CEL
