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Status
Public on Feb 01, 2007
Title
BM-MSC_rep2B
Sample type
RNA
Source name
human bone marrow-derived mesenchymal stem cell
Organism
Homo sapiens
Characteristics
Tissue: bone marrow-derived mesenchymal stem cell (Cambrex Lot#4F0312)
Biomaterial provider
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103, USA, [email protected] http://www.coriell.org/ Coriell
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated from cells in culture using the RNAeasy Mini Kit (Qiagen), according to manufacturer's protocol
Label
Biotin
Label protocol
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
Description
none
Data processing
RMA method implemented in BioConductor
Submission date
Oct 12, 2006
Last update date
Oct 13, 2006
Contact name
Kenro Kusumi
E-mail(s)
[email protected]
Phone
480-727-8993
Fax
480-965-6899
URL
http://sols.asu.edu/faculty/kkusumi.php
Organization name
Arizona State University
Department
School of Life Sciences
Street address
PO Box 874501
City
Tempe
State/province
AZ
ZIP/Postal code
85287
Country
USA
Platform ID
GPL97
Series (1)
GSE6029
Cord Blood-Derived Mesenchymal Stem Cells with Distinct Growth Kinetics, Differentiation Potentials, Expression Profiles
Data table header descriptions
ID_REF
VALUE
RMA expression estimate
Data table
ID_REF
VALUE
200000_s_at
8.30661521375268
200001_at
10.3889988896395
200002_at
11.0525642228058
200003_s_at
11.5071855143034
200004_at
10.524843526201
200005_at
9.30920372645267
200006_at
10.7007626984746
200007_at
10.5507284085411
200008_s_at
9.25580668900492
200009_at
10.411949373457
200010_at
10.8041061258104
200011_s_at
8.87305080824136
200012_x_at
11.5748588788455
200013_at
11.1151411620018
200014_s_at
9.33150152364213
200015_s_at
9.90135362867576
200016_x_at
10.7959607907526
200017_at
11.1013665268619
200018_at
11.5460234015855
200019_s_at
10.5762165555531
Total number of rows: 22645 Table truncated, full table size 602 Kbytes .Supplementary file
Size
Download
File type/resource
GSM139908.CEL.gz
3.0 Mb
(ftp) (http)
CEL