|
| Status |
Public on Oct 16, 2007 |
| Title |
Cochlea DBA2J 36 weeks 3 |
| Sample type |
RNA |
| |
|
| Source name |
RNA
|
| Organism |
Mus musculus |
| Characteristics |
Strain: DBA/2J mice
Gender: male
Age: 36-week-old
Tissue: cochlea
|
| Biomaterial provider |
JAX
|
| Treatment protocol |
To identify genes associated with AHL, each 7-week-old sample (n = 3) was compared to each 36-week-old sample (n = 3), generating a total of nine pairwise comparisons.
|
| Growth protocol |
All mice were raised under 12:12 dark/light conditions, and housed in the the University of Tokyo-approved Animal Care Facility, and were provided a nonpurified diet and acidified water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cochlea tissues using the TriZol reagent (Invitrogen) according to the manufacture’s instructions.
|
| Label |
Biotin
|
| Label protocol |
cDNA synthesis from total RNA (Invitrogen), cRNA amplification and labeling (Enzo BioArray IVT kit) were accomplished according to the manufacture’s instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization (Affymetrix Hybridization oven 640), washing, and staining (Affymetrix Fluidics station 400) were accomplished according to the manufacture’s instructions.
|
| Scan protocol |
Arrays were scanned on the Affymetrix GeneChip Scanner 3000. Image analysis was performed using the Affymetrix software.
|
| Description |
NA
|
| Data processing |
Scaled to 1500 (Affymetrix software)
|
| |
|
| Submission date |
Oct 16, 2006 |
| Last update date |
Oct 17, 2006 |
| Contact name |
Shinichi Someya |
| E-mail(s) |
[email protected]
|
| Phone |
608-265-5205
|
| Fax |
608-262-2976
|
| URL |
http://www.genetics.wisc.edu/faculty/profile.php?id=140
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Medical Genetics
|
| Lab |
Prolla Lab
|
| Street address |
425 Henry Mall Dr
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE6045 |
Gene expression profile of the age-related hearing loss cochlea in DBA/2J mice |
|
