|
| Status |
Public on Jun 04, 2014 |
| Title |
shEbf1 Hairpin 1_repeat2 |
| Sample type |
RNA |
| |
|
| Source name |
shEbf1 Hairpin 1
|
| Organism |
Mus musculus |
| Characteristics |
cell type: 3T3L1
lentivirus: shEbf1 Hairpin 1
|
| Treatment protocol |
Mature 3T3-L1 cells were subjected to 3-4 serial infections with virus, and selected with 5 μg/ml puromycin for at least 8 days prior to harvest.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
amplification with the NuGEN Ovation WTA Pico kit
|
| Label |
biotin
|
| Label protocol |
labeling with Encore Biotin Module.
|
| |
|
| Hybridization protocol |
As manufacturer's instruction
|
| Scan protocol |
As manufacturer's instruction
|
| Description |
shEbf1 Hairpin 1
|
| Data processing |
RMA procedure in BioConductor package for summarization and normalization
|
| |
|
| Submission date |
Jun 03, 2014 |
| Last update date |
Jun 04, 2014 |
| Contact name |
Evan Rosen |
| E-mail(s) |
[email protected]
|
| Organization name |
Beth Israel Deconess Medical Center
|
| Department |
Endocrinology
|
| Lab |
Rosen Lab
|
| Street address |
3 Blackfan Cir
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE58193 |
Characterizing the profiles of Ebf1 in mouse fat cells. |
|
