|
| Status |
Public on Jun 16, 2014 |
| Title |
SUP-HD1-FOXO1(3A)ER_4OHT_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
SUP-HD1-FOXO1(3A)ER, 4OHT, 24 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: cHL cell line
cell line: SUP-HD1
treatment: SUP-HD1-FOXO1(3A)ER cells incubated with 4OHT for 24 hours
|
| Treatment protocol |
KM-H2-KLF4ER and L428-KLF4ER cells were incubated with 200 µM 4-OHT or with relevant amount of the vehicle (ethanol). The experiment was done twice. After 24 h of incubation total RNA was isolated with RNeasy mini kit (QIAGEN).
|
| Growth protocol |
KM-H2, L428, L1236, UHO-1. and SUP-HD1 cHL cell lines stably expressing FOXO1(3A)ER were grown in RPMI1640 medium with addition of 10% FCS, glutamine and antibiotics at 37 ºC, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNeasy mini kit (QIAGEN) according to the manufacturers instruction.
|
| Label |
biotin
|
| Label protocol |
The total RNAs were amplified and labeled following the Whole Transcript (WT) Sense Target Labeling Assay (http://www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Labeled ssDNA was hybridized to Human Gene 1.0 ST Affymetrix GeneChip arrays (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
The chips were scanned with a Affymetrix GeneChip Scanner 3000 according to the Affimetrix protocol.
|
| Description |
SUP-HD1-FOXO1(3A)ER cells incubated with 4OHT for 24 hours
|
| Data processing |
The microarray data were processed using GeneSifter software (Perkin Elmers) using RMA normalization protocol.
|
| |
|
| Submission date |
Jun 16, 2014 |
| Last update date |
Jun 16, 2014 |
| Contact name |
Alexey Ushmorov |
| E-mail(s) |
[email protected]
|
| Phone |
+49 (0) 731 50033847
|
| Organization name |
Ulm University
|
| Department |
Institute of Physiological Chemistry
|
| Street address |
Albert-Einstein-Allee 11
|
| City |
Ulm |
| ZIP/Postal code |
89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE29545 |
Expression data of cHL cell lines after FOXO1 activation |
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