|
| Status |
Public on Feb 11, 2016 |
| Title |
Capan1_Vector_trans1 |
| Sample type |
RNA |
| |
|
| Source name |
Puromycin resistant Capan1 cells after transduction with MSCV-puro vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Capan1
cell type: Human pancreatic ductal adenocarcinoma
|
| Treatment protocol |
Capan1 cell were transduced with amphotropic retroviruses carrying either an MSCV-SV40-PuroR or MSCV-PROX1-PuroR, for 2.5 h. Three indeendent transductions were performed for each MSCV_puro and MSCV_PROX1_puro. Two days after transduction, the cells were incubated with 0.5 ug/mL puromycin and selected for 4 days. The selections was stopped after all the cells in the growth control (non-transduced Capan1) have died.
|
| Growth protocol |
Capan1 cells were grown in IMDM/FCS medium according to ATCC specifications.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol and the PureLink RNA Mini kit (Lifesciences).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 3’IVT Express protocol starting with 100 ng of total RNA (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
|
| |
|
| Hybridization protocol |
Six (6) micrograms of cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix GeneChip Primeview Human Gene Expression Array. Following hybridization, arrays were washed and stained using theAffymetrix Fluidics Station 450 system.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using Partek Genomics Suite software v6.5.
|
| |
|
| Submission date |
Jun 16, 2014 |
| Last update date |
Feb 12, 2016 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
[email protected]
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE58547 |
Prox1 haploinsufficiency sensitizes the pancreas to KrasG12D-induced transformation |
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