|
| Status |
Public on Aug 20, 2014 |
| Title |
Media 6h_1 |
| Sample type |
RNA |
| |
|
| Source name |
Media 6h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral blood monuclear cells
donor id: Healthy donor 1
|
| Treatment protocol |
Adherent peripheral blood monuclear cells from four healthy donors were stimulated with IL-10, IL-15 (R&D Systems), or IL-4 in RPMI 1640 supplemented with 10% fetal-calf serum. Cells were harvested at 6 h or 24 h after stimulation and monocytes purified by CD14 microbeads (Miltenyi Biotec).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol protocol for RNA isolation followed by purification of RNA by Qiagen Rneasy kit
|
| Label |
biotin
|
| Label protocol |
Performed by UCLA Clinical Microarray Core using Ambion Labeling kit
|
| |
|
| Hybridization protocol |
Performed by UCLA Clinical Microarray Core on GPL570
|
| Scan protocol |
Performed by UCLA Clinical Microarray Core
|
| Description |
A2
|
| Data processing |
Dchip analysis and RMA normalization using DNA-Chip Analyzer (Build date: Feb 21 2010, www.dchip.org)
|
| |
|
| Submission date |
Jul 08, 2014 |
| Last update date |
Aug 20, 2014 |
| Contact name |
Dennis Jay Montoya |
| E-mail(s) |
[email protected]
|
| Organization name |
UC Davis School of Medicine
|
| Department |
Biochemistry and Molecular Medicine
|
| Street address |
2700 Stockton Blvd #2354
|
| City |
Sacramento |
| State/province |
CA |
| ZIP/Postal code |
95817 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE59184 |
Microarray analysis adherent peripheral blood mononuclear cells stimulated with IL-10, IL-15, and IL-4 |
|
