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Sample GSM145053 Query DataSets for GSM145053
Status Public on Nov 21, 2006
Title I_U133Plus_59_AnCg
Sample type RNA
 
Source name Postmorten human brain: Anterior Cingulate
Organism Homo sapiens
Characteristics GeneChip_Assay_Site: I
Original_GeneChip_Type: U133Plus
Subject_ID: 59
Brain_Region: Anterior Cingulate
Redo: No
Cohort: 6
Sample_Block: 3
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133Plus Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Description Gene expression data from postmorten brain regions
Data processing The data were analyzed with the GCRMA method using custom CDF file Hs133A_Hs_UG_3.
 
Submission date Nov 16, 2006
Last update date Nov 20, 2006
Contact name Fan Meng
E-mail(s) [email protected]
Phone 734-615-7099
Organization name U of Michigan
Department Psychiatry
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
 
Platform ID GPL4557
Series (1)
GSE6306 Sample Matching by Inferred Agonal Stress in Gene Expression Analyses of the Brain

Data table header descriptions
ID_REF
VALUE GCRMA-calculated log2(Signal intensity) using custom CDF Hs133A_Hs_UG_3

Data table
ID_REF VALUE
AFFX-BioB-3_at 4.790700648
AFFX-BioB-5_at 6.118098617
AFFX-BioB-M_at 7.032221181
AFFX-BioC-3_at 7.892568841
AFFX-BioC-5_at 7.917640419
AFFX-BioDn-3_at 10.61879291
AFFX-BioDn-5_at 9.033696863
AFFX-CreX-3_at 12.95719067
AFFX-CreX-5_at 12.76785216
AFFX-DapX-3_at 2.318021514
AFFX-DapX-5_at 2.56786718
AFFX-DapX-M_at 2.50210603
AFFX-HSAC07/X00351_3_at 13.69289994
AFFX-HSAC07/X00351_5_at 9.737491398
AFFX-HSAC07/X00351_M_at 10.95222266
AFFX-hum_alu_at 14.47106119
AFFX-HUMGAPDH/M33197_3_at 13.54070716
AFFX-HUMGAPDH/M33197_5_at 12.05905295
AFFX-HUMGAPDH/M33197_M_at 12.55704427
AFFX-HUMISGF3A/M97935_3_at 8.853403531

Total number of rows: 12734

Table truncated, full table size 308 Kbytes.




Supplementary file Size Download File type/resource
GSM145053.CEL.gz 3.4 Mb (ftp)(http) CEL

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